Page 10 - 麻醉与监护论坛2015年第3期

Observation of Plasma Proendothelin-1 in 135 Patients
with Sepsis

Chang-xing Guo, Zhao-fen Lin, MORGENTHALER NILS G, KOHRLE JOSEF,
Xing-yi Yang, Liang Zhao, Hong-wei Shan, Yong-hua Xu, Qiang Zhang

Emergency Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China

Summary

Background: To evaluate the significance of proendothelin-1 (proET-1) in the diagnosis of patients with sepsis (bacterial infection), we determined
plasma ProET-1 levels in 135 patients with sepsis in the ICU of Shanghai Changzheng Hospital from January 2006 to June 2007.

Methods: Venous blood was collected from patients’ duringa 14 day period depending on the clinical condition. The blood specimens were
centrifuged and stored at -20℃. Sandwich ELISA was used to determine the proET-1 concentration in the plasma. All patients were divided into three
groups (healing group, improved group, unimproved group) according to the clinical response. The data were processed with SPSS13.0 software. The
relationship between proET-1 and clinical efficacy or infection score was analyzed.

Results: The results showed that proET-1 levels of the unimproved group were significantly higher than those of the healing group (p<0.001). The
level of ProET-1 in plasma was significantly associated with the clinical response. There was a positive correlation between them (p<0.001). A strong
relation was demonstrated between infection score and clinical efficacy, and there was also a positive correlation between the proET-1 levels and clinical
efficacy (p<0.001).

Conclusions: The results of the present study indicate that proET-1 varies with infection score and the clinical efficacy. Both have a positive
correlation and statistically significant difference. ProET-1 might serve as a sensitive marker of septic patients for early diagnosis of severe infections.

Key Words: Proendothelin-1; Sepsis; Early diagnosis
Corresponding Author: Zhao-fen Lin, E-mail: lin_zhaofen@126.com

INTRODUCTION tone and endothelial functions [3]. Nils G. Morgenthaler,
et al found that the plasma concentration of ET-1
Vascular endothelin-1 (ET-1), a 21-amino acid increases significantly in some clinical status such as
vasoactive peptide mainly produced by vascular sepsis, a serious disease with a high mortality rate. Stable
endothelial cells, is a potent angiotensin and involved proET-1 can be a reliable diagnostic marker as an indirect
in the regulation of vascular tone and smooth muscle assessment of ET-1 [4]. ProET-1 can be valuable for the
cell proliferation [1]. ET-1 was originally isolated from clinical diagnosis and treatment of sepsis. To evaluate the
the supernatant of cultured porcine aortic endothelial significance of proET-1 in the diagnosis of the patients
cells. Subsequently, ET-1 has been shown to possess with sepsis, sandwich immunoassays were used to
a number of different biological activities [2]. ET-1 determine circulating proET-1 content in plasma in 135
displays chemotactic properties on monocytes and is a cases of septic patients from January 2006 to June 2007
potent inducer of cell adhesion molecules in endothelial in the ICU of Changzheng hospital (The Second Military
cells. Furthermore, ET-1 is thought to act locally in a Medical University Shanghai) to evaluate the relationship
paracrine and autocrine fashion to regulate vascular

Laboratory and ClinicalCInovveesrtiTghaetsioisn 18 FAM 2013 Jan/Feb Vol.20 Issue 1