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between proET-1 and sepsis. With this approach we could the proET-1 level in the plasma. Other specimens from
test whether proET-1 content in plasma can be used as patients (such as sputum, etc) were collected for local
a reliable diagnostic marker which may help in the early secretion culture and routine blood test.
diagnosis of the sepsis.
The criteria of treatment efficacy
MATERIALS AND METHODS According to the principles of clinical antibiotics
study: (1) Healing: four criteria, including the symptoms,
Subjects signs, laboratory tests, and bacteriological examination
From January 2006 to June 2007, 135 cases were of the patient recovered to the normal. (2) Effective:
admitted to the ICU in Changzheng hospital including significant improvement of the disease, but one of four
100 males and 35 females with the average age of 63.35 criteria was not completely back to normal. (3) Improved:
±16.33 (17-80 years old). All the patients met the clinical condition improved after treatment, but not significantly.
diagnostic criteria for sepsis according to the following (4) Unimproved: all symptoms, physical signs of the
criteria: (1) histological, microbiological, and/or gross patients at the time of enrollment were maintained or
confirmation of infection, and (2) systemic illness worsened after 3 days of treatment.
caused by infection and had a positive blood culture of a Statistical method
pathogenic microorganism. All the data were processed by SPSS 13.0 software. The
Methods relation between the level of proET-1, and clinical data
The collection of venous blood was taken once a (clinical efficacy and infection score) was analyzed.
day from the first day to the third day after admission.
Beginning on the fourth day, venous blood was taken once RESULTS
every two days; 3-9 venous blood collections were taken
from the patients depending on each patient’s condition. According to the clinical response, 135 cases were
The observation time was 14 days if the patient survived
during this stage. All the blood samples were centrifuged divided into three groups: healing group (95 cases),
and stored at -20℃. A sandwich ELISA method was
used (provided by BRAHMS, Germany) to determine effective and improved group (7 cases), and unimproved

Table 4 The correlation between proET-1 content and clinical
efficacy.

Table 1 Patients’ characteristics at enrolment. Spearman's rho proET-1 Correlation Coefficient proET1 group
group Sig. (2-tailed) 1.000 0.175**
Group Number of patients (M/F) Mean Age (yrs) N 0.000
Healing group 95 (67/28) 61.8597 ±16.51840 967
Effective and improved group 7 (4/3) 61.0000 ±14.32946 Correlation Coefficient 0.175** 967
Unimproved group 33 (28/5) 67.7513 ±14.61004 Sig. (2-tailed) 0.000 1.000
N
967 977

** Correlation is significant at the 0.01 level (2-tailed).

Table 5 Infection score varies in three groups.

Table 2 ProET-1 content in three groups (nmol/L). Group Mean N Std. Deviation Std. Error of Mean
Healing group 9.6385 733 5.36600 0.19820
Group Mean N Std. Deviation Variation Effective and improved group 10.6000 45 4.63877 0.69151
Healing group 64.4094 730 64.54917 4166.595 Unimproved group 14.5816 196 7.32320 0.52309
Effective and improved group 70.0849 43 135.70907 18416.951 10.6776 974 6.10467 0.19561
Unimproved group 100.9884 194 81.70403 6675.548 Total
72.0002 967 74.10321 5491.286
Total ** Correlation is significant at the 0.01 level (2-tailed).

Table 3 The comparison of proET-1 in different groups. Table 6 The comparison of infection score in three groups.

Chi-Square (a,b) ProET1 Group Chi-Square(a,b) Infection Score Group
df 285.425 802.983 df 802.807 802.983
34
Asymp. Sig. 654 2 Asymp. Sig. .000 2
1.000 .000 .000

a. 655 cells (100.0%) have expected frequencies less than 5. The minimum expected cell frequency is 1.5. a. 0 cells (0%) have expected frequencies less than 5. The minimum expected cell frequency is 27.8.
b. 0 cells (0%) have expected frequencies less than 5. The minimum expected cell frequency is 325.7.
b. 0 cells (0%) have expected frequencies less than 5. The minimum expected cell frequency is 325.7.

Laboratory and Clinical ICnovveesrtigTahteiosins 19 FAM 2013 Jan/Feb Vol.20 Issue 1