Page 13 - 麻醉与监护论坛2015年第5期

Cover Thesis

P<0.05 means difference has statistically significant. according to the results in the concentration of 80pg/ml-
10ng/ml linear is good, the equation for: Y = 12.613 X -
Results 12.053, R2 = 0.9862, combined with the provisions of the
sensitivity of 45.42pg/ml, his own method to detect PCT
2.1 The Observation for Microsphere (Fig.1) linear range of 45.42pg/ml - 10ng/ml.
Microspheres showed good microspheres particle size
uniformity, high dispersibility, no gathered phenomenon, 2.4 Precision Test
shape rules, no abnormalities by electron microscopy to To use self-created method to detection the geriatric
preliminary observation, can be used to study biological trauma group mixed serum and control mixed serum, 20
molecular markers. times, record every MFI, looking for PCT concentration,
standard deviation and coefficient of variation. Geriatric
2.2 Sensitivity Test trauma group mixed serum the average of MFI is 56.43,
To use the self-created method to test the blank blank group MFI value is 4.54, streaming histogram as
samples and contains 500 pg/ml PCT standard, test 20 shown in fig.3, can calculate a geriatric trauma group
times, average, standard deviation, sensitivity. Self-created of mixed serum PCT concentrations of 10.42ng/ml, the
method measured blank sample, the MFI value is 4.54, standard deviation is 0.56ng/ml, the variation coefficient
and the standard deviation was 0.75; 500 pg/ml of PCT was 5.37%. For control group mixed serum, the average of
standard MFI value of 29.31, the standard deviation of MFI is10.52, serum PCT concentrations is 79.21pg/ml, the
5.17. 500 pg/ml of MFI value minus the blank sample standard deviation of 6.54pg/ml, the variation coefficient
MFI value of 24.77, according to the 99.7% confidence was 8.74%.
interval, blank samples 3 times the standard deviation of
0.75 * 3 = 2.25, as a result, when the MFI value is 2.25, 2.5 Positive Limit
that is equivalent to the detection sensitivity: 2.25/24.77 x To detection results in elderly patients with trauma MFI
500 = 45.42 pg/ml. is greater than the positive control group 2 times. The average
of MFI in the control group was 17.43, the positive value was
2.3 Linear Range (Fig.2) 17.43 * 2=34.86, and the Positive lower limit was 0.563ng/ml,
To use PCT standard concentration mixture different
standards (20ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1.25ng/ Table 1 Characteristics of trauma-induced old major trauma
ml, 625pg/ml, 315pg/ml, 160pg/ml, 80pg/ml), through
self-created method of detection of PCT levels, record patients and control patients
MFI values, the PCT level as the X, MFI has a value of Y,
Index Control group Low ISS group High ISS group
Fig .3 The cytofluorography histogram of self-created N 30 45 32
methods in PCT detection of old- patients
Age（year） 69.6±7.9 67.4±4.3 69.1±6.2
Man/woman 20/10 32/13 24/8
blunt injury 34 7
penetrating injury 11 25

ISS Score 20.7±0.9* 27.5±2.4
GCS Score 13.2±0.5* 9.6±0.5
APACHEⅡ Score 9.8±0.6* 16.9±0.9
mortality（%） 28.13
6.67 *

*: compared with high ISS group P < 0.01;

Tab.2 Comparison of APACHE Ⅱ, PCT, CRP between High-
ISS, Low-ISS and Control group

Group N 1d 3d 5d 7d 10d

High (APACHEⅡ) 32 16.9±0.9 19.3±2.1 23.6±1.9 18.4±2.6 14.8±3.1
Low 45 9.8±0.6* 16.3±2.2# 14.4±1.8* 11.3±1.3* 8.2±0.8*

Control (PCTng/ml) 30 0.21±0.07#

High 32 2.54±1.21 5.27±2.63 6.87±1.34 3.25±1.17 2.01±0.98
Low 45 0.86±0.47▽ 2.12±1.18▽ 1.71±0.62* 1.11±0.52* 0.78±0.37▽

Control (CRPmg/L) 30 4.3±2.1#

High 32 56.21±26.43 85.24±27.81 94.27±30.23 74.27±15.38 53.36±11.53
Low 45 23.17±9.52▽ 33.79±11.91* 41.95±16.73* 39.24±8.96* 37.58±8.50▽

▽: compared with high ISS group (P<0.05); *: compared with high ISS group (P<0.01);
#: compared with high and low ISS group (P<0.01)

Laboratory and Clinical CInovveesrtiTghaetisoins 94 FAM 2013 Mar/Apr Vol.20 Issue 2