Page 24 - 麻醉与监护论坛2016年第3期

李秀菊１ 王成夭２ 夏颖３ 咸淑悦２ 摘要
李心怡２ 马敏２ 潘霞２
目的：评价高迁移率族蛋白1(H M G B1)、S100B和糖基化终末产物受体(R A G E)的
1.武汉市第八医院麻醉科 湖北 武汉 430010 表达与大鼠脑缺血后认知功能障碍发生的关系。方法：雄性S D大鼠48只，3-4月龄，
2.武汉大学中南医院麻醉科 湖北 武汉 430071 体重250-300g，采用随机数字表法，将其分成2组(n=24)：假手术组(S H组)和脑缺血
3.湖北省妇幼保健院 湖北 武汉 430070 组(B I组)。通过阻断双侧颈总动脉(C C A)及椎动脉血流成功建立了四血管闭塞法大
鼠全脑缺血模型，分别于术后1、3、5和7d时，每组取6只大鼠，采用Y迷宫测试认
知功能，记录正确反应次数和全天总反应时间；分别于上述时点认知功能测试结束
后，处死3只大鼠，取海马组织，采用Western blot法测定海马CA1区HMGB1、S100B
和R A G E的蛋白表达；处死余3只大鼠，取脑组织，荧光共聚焦显微镜下观察H M G B1、
S100B和R A G E共表达情况。结果：与S H组比较，B I组术后1、3、5、7d认知功能明
显均出现减退，表现为站立次数和正确反应次数减少，全天总反应时间明显增加
(P<0.01)。与S H组比较，B I组大鼠海马H M G B1、S100B和R A G E蛋白表达在术后1d明显
增加(P<0.01)；术后7d，则基本恢复至对照组水平。免疫荧光实验显示HMGB1和RAGE
主要在海马CA1区呈锥体细胞层表达，S100B主要在海马CA1区呈放射性表达。与SH组
比较，BI组术后1、3和5d，大鼠海马CA1区HMGB1、S100B和RAGE阳性细胞计数显著增
加(P<0.01)；术后7d，基本恢复至正常对照组水平。结论：大鼠脑缺血后认知功能
障碍发生只可能与海马CA1区的HMGB1、S100B和RAGE表达上调有关。

关键词 脑缺血；认知障碍；高迁移率族蛋白质类；S100蛋白质类；糖基化终
末产物

HMGB1、S100B和RAGE的表达与大鼠脑
缺血后认知功能障碍发生的关系

Correlation Between HMGB1/RACE and S100B/RAGE
Expression and Cognitive Dysfunction of Rats Induced by Brain
Ischemia

LI Xiuju1,WANG Chengyao2, XIA Ying3,XIAN Shuyue2,LI Xinyi2,MA Min2,PAN Xia2

1.Dept.of Anesthesiology,Wuhan Eighth Hospital,Wuhan 430010,China
2.Dept.of Anesthesiology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China
3.Dept.of Anesthesiology,Hubei Maternal and Child Health Hospital,Wuhan 430070,China

Abstract

Objective: To evaluate the correlation between HMDB1/RACE and S100B/RAGE expression and congnitive dysfunction

of rats induced by brain ischemia.

Methods: Forty-eight male SD rats,aged 3-4 months,weighing 250-300g,were randomly divided into 2 groups(n=24

each):group sham operation(group SH)and group chronic brain ischemia(group BI).The brain ischemia models were made
by permanenr ligation of bilateral common carotid arteries.And cognitive function was assessed by using Y maze test on
the 3rd,5th and 7th day after operation.The expressions of HMGB1/RAGE,S100B/RAGE were detected by Western Bolt.The
colocalizations of HMGB1/RAGE,S100B/RAGE were observed by double immunofluorescent staining.

Results: Compared with group SH,group BI significantly decreased the number of grid cross,the rearing and correct

responses and increased the time the animals spent in the central square and total reaction as compared with group
SH(P<0.01).The HMGB1,S100 and RAGE expressions significantly increased on the 1st,3rd and 5th day after operation in group
BI as compared with group SH(P<0.01),and decreased to the level og group SH on the 7th day. The immunofluorescence
expression of HMGB1,S100B,RAGE and positive cells count showed that HMGB1 and RAGE were mainly expressed in pyramidal

Laboratory and Clinical Investigation 53 FAM 2016 Jan/Feb Vol.23 Issue 1
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