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王林1,2 童随阳1 刘浙波1 付金蓉1 摘要
曾彬1 夏豪1
目的：探讨整合素连接激酶(I L K)修饰的骨髓间充质干细胞(M S C s)
1.武汉大学人民医院心血管内科 湖北武汉 对心肌梗死后心脏心功能的影响。方法：取大鼠骨髓体外分离扩增培
430060 养MSCs，分别用ILK、ILK-siRNA及对照GFP腺病毒转染MSCs。结扎大鼠
2.十堰市郧阳区人民医院心血管内科 湖北十堰 心脏左前降支1h后，分别将GFP－MSCs、ILK-MSCs、ILK-siRNA-MSCs多
442500 点注射到大鼠心肌梗死区周边，对照组注射等量P B S。结果：细胞移植
４ｄ后与Ｇ Ｆ Ｐ－M S C s移植组相比，I L K-M S C s组存活细胞数目明显增
多，而I L K-s i R N A－M S C s移植组存活细胞明显减少(P<0.05)。细胞移植
３周后与GFP－MSCs移植组相比，ILK-MSCs组新生血管数目明显增多，
且胶原蛋白沉积减少，梗死面积明显缩小，心功能各项指标明显改善
(P<0.05)；而I L K-s i R N A－M S C s移植组新生血管数目明显减少，胶原蛋
白沉积增多，梗死面积明显增加，心功能各项指标明显恶化(P<0.05)。
结论：I L K增加M S C s存活率、促进梗死后心脏血管新生，从而抑制心室
重构，改善心功能。
关键词:骨髓间充质干细胞；心肌梗死；整合素连接激酶；血管再
生；生存
整合素连接激酶修饰的骨髓间充质干细胞
改善大鼠梗死后心脏心功能
Effect of Mesenchymal Stem Cells Transfected with Intergrin-
Linked Kinase Gene on Myocardial Function
WANG Lin1,2,TONG Suiyang1,LIU Zhebo1,FU Jinrong1,ZENG Bin1,XIA Hao1
1.Dept.of Vasculocardiology,Renmin Hospitsl of Wuhan University,Wuhan 430060,China
2.Dept.of Vasculocardiology,Yunyang Hospital of Shiyan,Shiyan 442500,Hubei,China
Abstract
Objective. To investigate whether intergrin-linked kinase(ILK)transduced by MSCs can imptove heart function after
heart infraction.
Methods. MSCs were acquired from the bone marrow of adult rats.The cells were isolated,purified,cultured,and
transfected with Adv-ILK,Adv-ILK-siRNA,and Adv-GFP respectively.1×106ILK-MSCs,GFP-MSCs,ILK-siRNA-MSCs or PBS was
respectively injected into rat hearts 1h intramyocardially after myocardial infarction.
Results. It was observed that ILK-overexpressed MSCs increased MSCs survival at 4 the end of days and angiogenesis
at the enf of 3 weeks after transplantation into infracted myocardium as compared with GFP-MSCs group and ILK-SiRNA-
MSCs group.This enhanced response was associated with attenuated left ventricular(LV)chamber dilation,reduced LV
fibrosis,decreased infarct size and improved LV function.
Conclusion. ILK modification with MSCs increases MSCs survival and angiogenesis,and preserves cardiac function and
inhibits remodeling.
Key Words. Mesenchymal Stem Cells;Myocardial Infarction;Intergrin-Linked Kinase;Angiogenesis;Survival
心肌梗死后受损区域心肌的血液循环中断，氧气、营养物 功能[2]。整合素连接酶激酶(intergrin-linked kinase,ILK))
质供应剧减导致大量心肌细胞丢失[1]；心肌梗死后心肌组织中 是一种机体内广泛分布的丝／苏氨酸蛋白激酶，通过磷酸化下
的急慢性炎症反应相继发生，炎症环境严重的限制了移植细胞 游底物P K B、G S K3β不但参与信号通路转导，调控着细胞的增
的存活及后续作用。抗凋亡、抗炎症反应虽能一定程度的提高 殖、分化、凋亡和存活，还可以促进血管新生。
移植细胞的综合生物学作用；但血管再生不仅可以提高移植细
胞的存活率而且可以减轻心肌凋亡，抑制心室重构、改善心脏 一、材料与方法
Laboratory RaenvdieCwlinaincdalCInMvEesLteigcatutiroen 40 FAM 2016 Jan/Feb Vol.23 Issue 1
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